Potential of avocado oil (Persea americana) in improving triglyceride and High Density Lipoprotein (HDL) levels in rats (Rattus novergicus) model of dyslipidaemia due to high fat diet

Eka Puspita Sari; Brian Wasita; Muthmainah Muthmainah

doi:10.21927/ijnd.2024.12(4).296-305

Potential of avocado oil (Persea americana) in improving triglyceride and High Density Lipoprotein (HDL) levels in rats (Rattus novergicus) model of dyslipidaemia due to high fat diet

Eka Puspita Sari, Brian Wasita, Muthmainah Muthmainah

Abstract

Background: Elevated levels of Dyslipidaemia pose a significant threat to cardiovascular health. Fortunately, the abundance of polyunsaturated and monounsaturated fatty acids (PUFA and MUFA) present in avocado oil offers a promising avenue for individuals with Dyslipidaemia to improve their lipid profile levels.

Objectives: The objective of this research is to ascertain the potential of avocado oil in improving triglyceride and HDL levels.

Methods: A pre-post test control group design was employed, utilizing male Wistar rats aged 8 weeks with a body weight ranging from 150-200 g. The mice were segregated into six groups: N, K-, K+, P1, P2, and P3. Throughout the trial, solely group N abstained from ingesting avocado oil or HC. Conversely, all other groups were administered HC and subsequently, K+ was prescribed simvastatin while P1, P2 and P3 received avocado oil for a duration of 28 days. The data was subjected to statistical analysis using SPSS vr.25. The statistical significance threshold was set at p<0.05.

Results: Mean changes in levels (mg/dL) for TG -29.67 (P1), -48.99 (P2), -56.78 (P3) and for HDL 41.75 (P1), 46.35 (P2). There was a significant difference in TG and HDL levels between P1, P2, P3 when compared with K- (p<0.05).
Conclusion: Avocado oil exhibits efficacy in lowering TG levels and elevating HDL levels, rendering it a viable option for daily consumption.

Keywords

avocado oil, Dyslipidemia, Triglyceride (TG), High Density Lipoprotein (HDL)

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References

Supariasa IDN, Handayani D. Asuhan Gizi Klinik. 1st ed. Jakarta: EGC; 2019. 1–500 p.

WHO. Cardiovascular diseases (CVDs). 2017.

Carroll MD, Fryar CDH. Total and High-density Lipoprotein Cholesterol in Adults: United States, 2015-2018. NCHS Data Brief. 2020;(363):2015–8.

Lin CF, Chang YH, Chien SC, Lin YH, Yeh HY. Epidemiology of Dyslipidemia in the Asia Pacific Region. International Journal of Gerontology. 2018;12(1):2–6.

PERKENI. Pedoman Pengelolaan Dislipidemi di Indonesia 2019. 1st ed. M AMA, Soewondo P, Soelistijo SA, Arsana PM, Wismandari, Zufry H, et al., editors. PB. Perkeni. PB Perkeni; 2019. 9 p.

Grundy SM, Stone NJ, Bailey AL, Beam C, Birtcher KK, Blumenthal RS, et al. Guideline on the Management of Blood Cholesterol: A Report of the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines. Jornal of the American College of Cardiology. 2019;73(24):e285–350.

PERSAGI, ASDI. Penuntun Diet Dan Terapi Gizi. 4th ed. Suharyati, Hartati B, Kresnawan T, Sunarti, Hudayani F, Darmariini F, editors. Jakarta: EGC; 2019. 403 p.

Hasibuan Putir. Evaluasi Penggunaan Obat Dislipidemia Rawat Jalan di Rumah Sakit Umum Daerah Dr. Pirngadi Kota Medan. Repository Usu. Universitas Sumatera Utara; 2018.

BPS. Produksi Tanaman Buah-buahan Tahunan. In: https://www.bps.go.id/indicator/55/62/3/produksi-tanaman-buah-buahan.html. 2021.

Brown AC. Understanding Food Principles and Preparation. 6th ed. Variety. Amerika Serikat: Cengage Learning; 2018. 704 p.

Rahardjo AP, Manaf Y, Ambarita M, Nusantoro B. Minyak Goreng Untuk Pengolahan Pangan. 1st ed. Siti, editor. yogyakarta: Gadjah Mada University Press; 2021. 216 p.

Tan CX. Virgin avocado oil: An emerging source of functional fruit oil. Journal of Functional Foods. 2019;54(October 2018):381–92.

Sutanto EY. Review Perkembangan Pedoman Diet Dan Penemuan Mengenai Efek Konsumsi Lemak Terhadap Kesehatan Kardiovaskular. University Katholik Soegijapranata Semarang; 2022.

Nasef ANZ, Ahmed AH. Effect of Bioactive Compounds of Avocado (Persea Americana) Fruit Powder on Hypercholesterolemic Rats: Biological and Biochemical Studies. Alexandria Sci Exch J. 2019;40:550–9.

Octavio Rodiles-López J, González-Montoya M, Eduardo Martínez-Flores H, Zamora-Vega R. The Effect of Diets Enriched with Avocado (Persea americana cv Hass) on Cholesterol and Triglyceride Decrease Evaluated in Hamsters. Journal of Food and Nutrition Research. 2020;8(11):670–4.

Tan CX, Chong GH, Hamzah H, Ghazali HM. Hypocholesterolaemic and hepatoprotective effects of virgin avocado oil in diet-induced hypercholesterolaemia rats. International Journal of Food Science and Technology. 2018;53(12):2706–13.

Tan CX, Chong GH, Hamzah H, Ghazali HM. Effect of virgin avocado oil on diet-induced hypercholesterolemia in rats via 1H NMR-based metabolomics approach. Phytotherapy Research. 2018;32(11):2264–74.

Ellen M, Khardori R. Hypertriglyceridemia. In: Medscape. 2021. p. 1–39.

Untari MK, Pramukantoro GE. Aktivitas Antihiperkolesterolemia Ekstrak Etanol Daun Stevia Rebaudiana Bertoni Pada Tikus Putih Jantan. Journal Syifa Sciences and Clinical Research. 2020;2(1):11–20.

Y MI, Montana C, Malik F, Susanty S. Penurunan Kadar Trigliserida Tikus Putih Wistar Jantan (Rattus novergicus) Hiperlipidemik Yang Diberi Ekstrak Terpuifikasi Batang Galing (Cayratia trifolia L. Domin.). Medical Sains. 2020;4(2):79–86.

Ihedioha JI, Noel-Uneke OA, Ihedioha TE. Reference values for the serum lipid profile of albino rats (Rattus norvegicus) of varied ages and sexes. Comparative Clinical Pathology. 2013;22(1):93–9.

Kodariah L, Wahid AA. Pengaruh Ekstrak Biji Ketumbar (Coriandrum Sativum) Terhadap Kadar Trigliserida Dan Gambaran Histologi Hati Tikus (Rattus Novergicus) Yang Diinduksi Oleh Pakan Tinggi Lemak. Jurnal Biotek Medisiana Indonesia. 2020;9(1):47–54.

Wahjuni S. Dislipidemia Menyebabkan Stress Oksidatif Ditandai Oleh Meningkatnya Malondialdehid. 1st ed. Utama IH, editor. Denpasar: Udayana University Press; 2015. 95 p.

Borén J, Taskinen M. Metabolism of Triglyceride-Rich Lipoproteins. In: Prevention and Treatment ofAtherosclerosis, Handbook of Experimental Pharmacology. 2021. p. 133–56.

Lent-Schochet D, Jialal I. Biochemistry, Lipoprotein Metabolism. PMID:31985. StatPearls; 2023.

Cao X, Xia J, Zhou Y, Wang Y, Xia H, Wang S, et al. The Effect of MUFA-Rich Food on Lipid Profile: A Meta-Analysis of Randomized and Controlled-Feeding Trials. Foods. 2022;11(13).

Jusup I. Pengaruh Vitamin E Dan Olahraga Terhadap Stres Oksidatif: Studi Pada Mencit Yang Terpapar Minyak Goreng Berulang. Jnh. 2014;2(3).

Carvajal-Zarrabal O, Nolasco-Hipolito C, Aguilar-Uscanga MG, Melo-Santiesteban G, Hayward-Jones PM, Barradas-Dermitz DM. Avocado oil supplementation modifies cardiovascular risk profile markers in a rat model of sucrose-induced metabolic changes. Disease Markers. 2014;

Sari EP, Wasita B, Muthmainah. The Role Of Avocado Oil As Anti-Dyslipidemia And Hepatoprotectiv. Al Insyirah International Scientific Conference on Health. 2024;129–38.

Lukas Schwingshackl, Jasmin Zähringer, Jessica Beyerbach, Sarah S. Werner, Blin Nagavci, Helmut Heseker, Berthold Koletzko, Joerg J. Meerpohl, on behalf of the International Union of Nutritional Sciences (IUNS) Task force on Dietary Fat Quality; A Scoping Review of Current Guidelines on Dietary Fat and Fat Quality. Annals of Nutrition and Metabolisme 12 August 2021; 77 (2): 65–82. https://doi.org/10.1159/000515671

DOI: http://dx.doi.org/10.21927/ijnd.2024.12(4).296-305