Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)

Rahma Micho Widyanto; Rifanty Meydiana Rachmawati Putri; Fuadiyah Nila Kurniasari; Yunimar Yunimar; Budi Utomo

doi:10.21927/ijnd.2019.7(2).51-57

Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)

Rahma Micho Widyanto, Rifanty Meydiana Rachmawati Putri, Fuadiyah Nila Kurniasari, Yunimar Yunimar, Budi Utomo

Abstract

ABSTRAK

Latar belakang: Kanker serviks merupakan penyakit yang menduduki posisi kedua penyebab kematian pada wanita. Berbagai terapi pendukung mulai dikembangkan, seperti melalui bahan makanan yang dipercaya memiliki efek anti-kanker. Buah sirsak disebut memiliki kandungan fitokimia seperti Annonaceous acetogenin, flavonoid dan fenol yang bermanfaat sebagai anti-kanker.

Tujuan: Penelitian ini dilakukan untuk mengetahui potensi anti-oksidan dan sitotoksisitas dari sari buah sirsak pada sel HeLa secara in vitro.

Metode: Penelitian ini dilakukan dengan membuat sari buah sirsak dengan cara diblender kemudian dikeringkan dengan metode freeze-drying, yang kemudian dilanjutkan dengan uji 2,2-diphenyl-1-picrylhydrazyl (DPPH) untuk mengetahui potensi penghambatan radikal bebas, dan potensi sitotoksisitas melalui uji MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difenitetrazolium bromide) assay pada sel kanker serviks HeLa.

Hasil: Hasil uji aktivitas anti-oksidan menunjukkan persamaan regresi linier (y=0,0149x – 2,8812) dan nilai perhitungan IC₅₀ sari buah sirsak sebesar 3549 μg/mL dan hasil uji sitotoksisitas menunjukkan persamaan regresi linier (y=0,0197x + 0,3101) dan nilai perhitungan IC₅₀ sari buah sirsak pada sel HeLa sebesar 2522,33 μg/mL.

Kesimpulan: Sari buah sirsak memiliki aktivitas anti-oksidan yang sangat rendah dan tidak berpotensi sebagai anti-kanker terhadap sel HeLa secara in vitro.

KATA KUNCI : Annona muricata Linn, anti-oksidan, kanker, sitotoksisitas

ABSTRACT

Background: Cervical cancer is the second leading cause of death in women. Various supporting therapies have been developed, such as through food ingredients which are believed to have anti-cancer effects. Soursop is known to be high phytochemical content such as Annonaceous acetogenin, flavonoid and phenols which are useful as anti-cancer.

Objectives: This research was conducted to determine the antioxidant and cytotoxic activity of soursop juice on HeLa cell lines.

Methods: This study started by making the soursop fruit extract by blending then dried with freeze-drying method, and then proceed with 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine the potential free radical scavenging activity, cytotoxic potential activity in vitro through MTT (3-(4,5-dimethylthiazol-2-il)-2,5-difenitetrazolium bromide) assay on HeLa cell lines.

Results: The IC₅₀ antioxidant activity of soursop fruit extract is 3549 μg/mL with linear regression equation (y=0.0149x – 2.8812) and the IC₅₀ cytotoxicity test of soursop fruit extracton HeLa was 2522,33 μg/mL with linier regression equation (y=0.0197x + 0.3101).

Conclusion: The conclusion in this study is that soursop fruit extract has very low antioxidant activity and has no in vitro potential effect as an anti-cancer on HeLa cell lines.

KEYWORDS : Annona muricata Linn, antioxidant, cancer, cytotoxic

Keywords

cancer, Annona muricata Linn., antioxidant, cytotoxic

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DOI: http://dx.doi.org/10.21927/ijnd.2019.7(2).51-57