Effect of loloh sembung (Blumea balsamifera) maturity stage on antioxidant activity

I Gusti Ayu Wita Kusumawati, I Nengah Reyunika, Ida Bagus Agung Yogeswara, I Gede Mustika, I Made Wisnu Adi Putra, Umar Santoso, Yustinus Marsono

Abstract


ABSTRAK

Latar belakang: Loloh sembung (Blumea balsamifera) adalah minuman tradisional masyarakat Bali yang digunakan untuk mengobati penyakit. Perbedaan kematangan daun sembung akan mempengaruhi aktivitas antioksidan loloh sembung.

Tujuan: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan loloh sembung yang dibuat dari berbagai jenis kematangan daun sembung yang diekstrak dengan menggunakan pelarut air.

Metode: Serbuk daun sembung pada tingkat kematangan yang berbeda (muda, dewasa dan tua) direbus untuk menghasilkan loloh sembung. Analisis kandungan antioksidan meliputi analisis total fenolik, total kandungan flavonoid dan aktivitas antioksidan ferric reducing antioxidant power (FRAP).

Hasil: Hasil analisis menunjukkan bahwa daun sembung dengan tingkat kematangan tua menunjukkan kandungan total fenolik dan aktivitas antioksidan yang tinggi dibandingkan dengan daun dewasa dan muda, yaitu masing-masing sebesar 0,85±0,005 GAE/g dan 0,66 ±0,003 mmol Fe2+/g sampel. Sedangkan, daun dengan tingkat kematangan dewasa menunjukkan total flavonoid yang tinggi, yaitu sebesar 0,39±0,006 QE/g. Berdasarkan korelasi Pearson, perbedaan tingkat kematangan daun menunjukkan korelasi positif dengan kandungan total fenolik, total flavonoid dan aktivitas antioksidan (FRAP).

Kesimpulan: Perbedaan tingkat kematangan daun sembung menunjukkan korelasi positif dengan kandungan total fenolik, total flavonoid dan aktivitas antioksidan (FRAP). Penelitian ini menunjukkan bahwa loloh sembung berpotensi sebagai minuman fungsional yang memanfaatkan kearifan lokal.

KATA KUNCI: tingkat kematangan daun, total fenolik, total flavonoid, FRAP

 

ABSTRACT

Background: A Loloh sembung (Blumea balsamifera) is a traditional herbal drink from Bali and widely used to treat several diseases by Balinese people. Sembung leaf at different maturity stages would affect antioxidant activity of loloh sembung.

Objectives: The objective of the research was determined antioxidant activity of loloh sembung prepare from different maturity stages of sembung leaf and extracted using water.

Methods: Sembung leaves powder at different maturity stages (young, mature and old) was boiled to produce loloh sembung. The analyses of antioxidant activity of loloh sembung included total phenolic content (TPC), total flavonoid content (TFC) and ferric reducing antioxidant power (FRAP).

Results: The results showed that old leaves were significantly higher in TPC and FRAP values compare to mature and young leaves, i.e 0.8575±0.005 GAE/g and 0.6625±0.003 mmol Fe2+/g sample respectively. However, the mature leaves revealed significantly high TFC, i.e 0.3972±0.006 QE/g. Based on Pearson’s correlation coefficient, the difference of maturity stage exhibited positive correlation with TPC, TFC and FRAP.

Conclusion: The different of maturity stage exhibite showed positive correlation with TPC, TFC and FRAP. This study suggested that loloh sembung had a promising prospect as functional drink based on local wisdom.

KEYWORDS: leaf maturity stage, loloh sembung, total flavonoids, total phenolic (italic)


Keywords


tingkat kematangan daun, total fenolik, total flavonoid, FRAP; leaf maturity stage, loloh sembung, total flavonoids, total phenolic (italic)

Full Text:

PDF

References


De Boer, H.J., and Cotingting, C. 2014. Review: Medical plants for women’s healthcare in southeast Asia: A meta-analysis of their traditional use, chemical constituents, and pharmacology. Journal of ethnopharmacology, 151: 747-767.

See, G.L.L., Arce, F.V.Jr., Deliman., and Yolanda C. 2016. ACE (Angiotensin Converting Enzyme) inhibition activity of oven-dried and air-dried sambong Blumea balsamifera L.(dc.) Tea. International Journal of Pharmacognosy and Phytochemical Research, 8(7): 1132-1136.

Kusumawati, I.G.A.W and Yogeswara,I.B.A. 2016. Antioxidant capacity and antibacterial of loloh sembung based on extraction methode. Medicine Traditional Journal, 21(3): 143-148.

Ali, D.M.H., K.C. Wong., and P.K. Lim. 2005. Flavonoids from Blumea balsamifera. Fitoterapia 76 : 128–130.

Yun D, Gaiyun Y, Jin Y, Bingjun Q, Zhenmin L, Danfeng W, Yun Z ,and Yanyun Z. 2014. Influences of ripening stages and extracting solvents on the polyphenolic compounds, antimicrobial and antioxidant activities of blueberry leaf extracts. Food Control, 38: 184-191.

Norlham.A, Dian N, Kherni, Nooraaln, Asisah, 2015. Influences of Maturity Stages and Extraction Solvents on Antioxidant Activity of Cosmos Caudatus Leaves. International Journal of Research Studies in Biosciences (IJRSB), 3(12): 1-10.

Morales, P., Helayne, A.M., Maria, I.D., Ricardo, C.C., Maria C.S-M., Celestino, S-B., Lillian, B., and Isabel, C.F.R.F. 2017. Hovenia dulcis Thunb. Pseudofruits as functional foods: Phytochemicals and bioactive properties in different maturity stages. Journal of Functional Foods, 29:37-45.

Gurjar, M. S., Ali, S., Akhtar, M., and Singh, K.S. 2012. Efficacy of plant extracts in plant disease management. Agricultural Sciences, 3 : 425-433.

Liu, L., Xiaolu C., Quan Y and Yuxin P. 2016. Morphological development of Sambong (Blumea balsamifera (L.) DC.) leaf studied by frozen section and thin section. Journal of Biosciences and Medicines, 4:10-13. http://dx.doi.org/10.4236/jbm.2016.44002.

Konczak,I., Dimitros,Z.,matthew, D., and Patricia A., 2010. Antioxidant capacity and phenolic compounds in commercially grown native australian herbs and species. food chemistry, 122: 260-266.

Ammar, I. Monia E., and Hamadi A. 2015. Phenolic content and antioxidant activity of cactus (Opuntia ficus-indica L.) flowers are modified according to the extraction method. Industrial crops and products. 64 : 97-104

Chang, C.C., Yang, M.H., Wem, H.M., and Chern, J.C. 2002. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods, Journal of Food and Drug Analysis, 10, 3 : 178-182

Choluj,D., R. Karwowska, M. Jasińska, and G. Haber. 2004. Growth and dry matter partitioning in sugar beet plants (Beta vulgaris L.) under moderate drought. Plant Soil Environ., 50 (6): 265–272.

Kusumawati, I.G.A.W., Darmawijaya, I.P., and Yogeswara, I.B.A. 2014. Potensi antioksidan loloh tempuyung (Sonchus arvensis L.) sebagai minuman fungsional. Prosiding Seminar Nasional Biologi FMIPA UNHI.

Maieves, H.A., Lopez-Froilan, R., Morales, P., Perez-Rodriguez, M.L., Ribani, R.H., Camara, M. 2015. Antioxidant phytochemicals of Hovenia dulcis Thunb. Peduncles in different maturity stage. Journal of functional foods, 18: 1117-1124.

Oloyede F.M, Oloyede F. A. And Obuotor E. M, 2012. Effect of Plant Maturity on the Antioxidant Profile of Amaranthus cruentus L. and Celosia Argentea L.. Bulletin of Environment, Pharmacology and Life Sciences, 2(2): 2277-1808.

Kamal U, Abdul S.J, Md. Eaqub A and Mohd R.I. 2012. Evaluation of Antioxidant Properties and Mineral Composition of Purslane (Portulaca oleracea L.) at Different Growth Stages. Molecular Sciences, Volume 13, pp. 1422-0067.

Abdulaziz Rabiu Abdulkadir, Md. Sarwar Jahan and Dhiya Dalila Zawawi, 2015. Effect of chlorophyll content and maturity on total phenolic, total flavonoid contents and antioxidant activity of Moringa oleifera leaf (Miracle tree). Journal of chemical an

d pharmaceutical reseach, 7(5), pp. 1147-1152.

Bini W,, Qingyuan H, Chandrasekar V, Hongkang C, Hui G, Ni C, Wei C, Xingang L, and Zhongli ., 2016. Changes in phenolic compounds and their antioxidant capacities in jujube (Ziziphus jujuba Miller) during three edible maturity stages. LWT - Food Science and Technology, 66(1), pp. 56-62.

Carocho, M and Ferreira, I.C.F.R. 2013. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food and chemical toxicology, 51: 15-25.

O'Byrne DJ, Devaraj S, Grundy SM, and Jialal I. 2002. Comparison of the antioxidant effects of Concord grape juice flavonoids alpha-tocopherol on markers of oxidative stress in healthy adults. American Journal Clinical Nutrition 76(6): 1367-1374.

Bae, H. et al., 2012. Extraction efficiency and validation of an HPLC method for flavonoid analysis in peppers. Food Chemistry, 130(3), pp.751–758. Available at: http://dx.doi.org/10.1016/j.foodchem.2011.07.041.

Biesaga, M., 2011. Influence of extraction methods on stability of flavonoids. Journal of Chromatography A, 1218(18), pp.2505–2512. Available at: http://dx.doi.org/10.1016/j.chroma.2011.02.059.




DOI: http://dx.doi.org/10.21927/ijnd.2018.6(1).1-6

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian Journal of Nutrition and Dietetics (IJND) indexed by:

  


Lisensi Creative Commons
View My Stats