Free radical scavenging and cytotoxic assay of soursop fruit juice (Annona muricata Linn.) on cervical cancer cell lines (HeLa)
DOI:
https://doi.org/10.21927/ijnd.2019.7(2).51-57Keywords:
cancer, Annona muricata Linn., antioxidant, cytotoxicAbstract
ABSTRAK
Latar belakang: Kanker serviks merupakan penyakit yang menduduki posisi kedua penyebab kematian pada wanita. Berbagai terapi pendukung mulai dikembangkan, seperti melalui bahan makanan yang dipercaya memiliki efek anti-kanker. Buah sirsak disebut memiliki kandungan fitokimia seperti Annonaceous acetogenin, flavonoid dan fenol yang bermanfaat sebagai anti-kanker.
Tujuan: Penelitian ini dilakukan untuk mengetahui potensi anti-oksidan dan sitotoksisitas dari sari buah sirsak pada sel HeLa secara in vitro.
Metode: Penelitian ini dilakukan dengan membuat sari buah sirsak dengan cara diblender kemudian dikeringkan dengan metode freeze-drying, yang kemudian dilanjutkan dengan uji 2,2-diphenyl-1-picrylhydrazyl (DPPH) untuk mengetahui potensi penghambatan radikal bebas, dan potensi sitotoksisitas melalui uji MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difenitetrazolium bromide) assay pada sel kanker serviks HeLa.
Hasil: Hasil uji aktivitas anti-oksidan menunjukkan persamaan regresi linier (y=0,0149x – 2,8812) dan nilai perhitungan IC50 sari buah sirsak sebesar 3549 μg/mL dan hasil uji sitotoksisitas menunjukkan persamaan regresi linier (y=0,0197x + 0,3101) dan nilai perhitungan IC50 sari buah sirsak pada sel HeLa sebesar 2522,33 μg/mL.
Kesimpulan: Sari buah sirsak memiliki aktivitas anti-oksidan yang sangat rendah dan tidak berpotensi sebagai anti-kanker terhadap sel HeLa secara in vitro.
KATA KUNCI : Annona muricata Linn, anti-oksidan, kanker, sitotoksisitas
ABSTRACT
Background: Cervical cancer is the second leading cause of death in women. Various supporting therapies have been developed, such as through food ingredients which are believed to have anti-cancer effects. Soursop is known to be high phytochemical content such as Annonaceous acetogenin, flavonoid and phenols which are useful as anti-cancer.
Objectives: This research was conducted to determine the antioxidant and cytotoxic activity of soursop juice on HeLa cell lines.
Methods: This study started by making the soursop fruit extract by blending then dried with freeze-drying method, and then proceed with 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay to determine the potential free radical scavenging activity, cytotoxic potential activity in vitro through MTT (3-(4,5-dimethylthiazol-2-il)-2,5-difenitetrazolium bromide) assay on HeLa cell lines.
Results: The IC50 antioxidant activity of soursop fruit extract is 3549 μg/mL with linear regression equation (y=0.0149x – 2.8812) and the IC50 cytotoxicity test of soursop fruit extracton HeLa was 2522,33 μg/mL with linier regression equation (y=0.0197x + 0.3101).
Conclusion: The conclusion in this study is that soursop fruit extract has very low antioxidant activity and has no in vitro potential effect as an anti-cancer on HeLa cell lines.
KEYWORDS : Annona muricata Linn, antioxidant, cancer, cytotoxic
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